Figure 5.
Rapid and efficient response of nonpolarized T cells to antigen in vivo. CFSE-labeled naive T cells (left) or nonpolarized T cells (right) were transferred into syngeneic normal mice. After 24 h, the mice were immunized. (A–D) Mice were challenged by subcutaneous administration of HA110–119 in CFA and cells from draining lymph nodes were analyzed after 48 h (A and B) or 5 d (C and D). (E and F) Mice were challenged by subcutaneous administration of HA110–119 in PBS and cells were analyzed after 5 d. (G and H) Mice were challenged by intranasal administration of HA110–119 in PBS and the cells from draining lymph nodes were analyzed after 5 d. The dot plots show division of CFSE-labeled cells vs. CD3 expression or vs. IFN-γ production after stimulation with PMA and ionomycin. One representative experiment out of three.