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. 2001 Apr 16;193(8):925–934. doi: 10.1084/jem.193.8.925

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Western blot for expression of SOD1, SOD2, and porin in RBCs and bone marrow. 50 μg of total protein lysate from RBCs or bone marrow cells of transplanted animals was separated on a 12% SDS gel and blotted for protein expression. Red cell lysates contain abundant SOD1, but do not contain detectable amounts of SOD2. The mitochondrial protein porin is present in bone marrow cells, and can be detected in the RBC lysate of Sod2 ^−/− transplant recipients. In a lighter exposure (*) of bone marrow–derived protein, porin is also more abundant in the sample from Sod2 ^−/− transplant recipients. SOD2 can be detected in the bone marrow lysates, and with a lighter exposure (**) expression levels can be correlated with the genotype of transplanted cells: Sod2^+/+ lysates express the most, Sod2^+/− lysates express less, and Sod2^−/− lysates have <5% the level of SOD2 protein seen in Sod2^+/+ samples. Results shown for porin and SOD2 expression are representative of four separate determinations.