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. 2001 Mar 19;193(6):769–776. doi: 10.1084/jem.193.6.769

Figure 2.

Figure 2

Figure 2

Figure 2

E2A and HEB promote TCR-δ rearrangement in BOSC 23 cells. (A) Schematic representation of TCR δ locus. (B) BOSC 23 cells were transfected with expression vectors for E12, E47, HEB, RAG1, and RAG2. 100 ng of DNA was amplified by PCR for Vδ2-Dδ3 recombination using the Vδ2 and Dδ3 primers. PCR products were separated on a 1.5% agarose gel, transferred to Nytran Plus, and hybridized with a Vδ2 probe. The REM cell line contains endogenous Vδ2-Dδ3 rearrangements. (C) Transfected BOSC 23 cells were analyzed by PCR for Vδ2-Dδ2 rearrangement. PCR products were separated by agarose gel electrophoresis, transferred to Nytran Plus, and hybridized with a Vδ2 probe. (D) Genomic DNA was analyzed with primers to the CD14 locus to demonstrate that all samples contained comparable amounts of DNA.