Table 2.

Sequences of Vγ-Jγ Coding Joints in Transfected BOSC 23 Cells

Vγ			Jγ2.3	V segment used
ACC TGG GAT AGG		G	AAT TAT TAT AAG
ACC TGG GAC AGG	C	–	––T TAT TAT AAG	V3
ACC TGG GAC AGG	C	–	––– ––T TAT AAG	V3
ACC TGG GAC AGG		–	–AT TAT TAT AAG	V7
ACC TGG GAT GGG	C	–	––T TAT TAT AAG	V4
ACC TGG GAT GGG	C	–	–AT TAT TAT AAG	V4
ACC T–– ––– –––		–	AAT TAT TAT AAG	V8
ACC TGG GAC AGG		–	–AT TAT TAT AAG	V7
ACC TGG GAT GGG	CC	–	gAT TAT TAT AAG	V4
ACC TGG GA– –––		G	AAT TAT TAT AAG	V7
ACC TGG GAT GGG	C	–	––T TAT TAT AAG	V4
ACC TGG GAC AGG		–	––– –AT TAT AAG	V7
ACC TGG GAT GGG	CC	–	gAT TAT TAT AAG	V4
ACC TGG GAC –––		–	AAT TAT TAT AAG	V7
ACC TGG GAC AGG		–	–AT TAT TAT AAG	V7
ACC TGG GAC AGG		–	––– –AT TAT AAG	V7
ACC T–– ––– –––		–	AAT TAT TAT AAG	V8
ACC TGG GAT GGG	CC	–	gAT TAT TAT AAG	V4
ACC TGG GAT AG–		G	AAT TAT TAT AAG	V8
ACC TGG GAT GGG	C	–	––T TAT TAT AAG	V4
ACC TGG GAC AGG		–	–AT TAT TAT AAG	V7
ACC TGG GAT AG–		–	AAT TAT TAT AAG	V8
ACC T–– ––– –––		–	AAT TAT TAT AAG	V8

Coding joints in BOSC 23 cells transfected with either E2A or HEB and RAG1 and RAG2, are diverse and resemble those found in T lineage cells. Genomic DNA from transfected cells was amplified with the VγI family and Jγ1.3/2.3 primers. The resulting fragments were cloned and sequenced. Dashes represent bases that have been deleted. Underlined bases indicate P nucleotide additions. Bases in lowercase lettering could not be matched to the germline DNA sequence.