Table 3.

Sequences of Vδ-Dδ Coding Joints in Transfected BOSC 23 Cells

Vδ2		Dδ3
GCC TGT GAC ACC		ACT GGG GGA TAC G
GCC TGT GAC AC–		–CT GGG GGA TAC G
GCC TGT GAC –––		ACT GGG GGA TAC G
GCC TGT GAC AC–		–CT GGG GGA TAC G
GCC TGT GAC ACC		ACT GGG GGA TAC G
GCC TGT GAC –––		ACT GGG GGA TAC G
GCC TGT GAC ACC		––T GGG GGA TAC G
GCC TGT GAC –––		ACT GGG GGA TAC G
GCC TGT GAC ACC		–CT GGG GGA TAC G
GCC TGT GAC AC–		–CT GGG GGA TAC G
GCC TGT GAC –––		ACT GGG GGA TAC G
GCC TGT GAC ACC		––– –GG GGA TAC G
GCC TGT GAC ACC		––T GGG GGA TAC G
GCC TGT GAC ACC		–CT GGG GGA TAC G
GCC TGT GAC –––		–CT GGG GGA TAC G
GCC TGT GAC –––		ACT GGG GGA TAC G
GCC TGT GAC ACC	G t	––T GGG GGA TAC G
GCC TGT GAC ACC		––T GGG GGA TAC G
GCC TGT GAC –––		––– ––– ––– –AC G

DNA sequences of Vδ2-Dδ3 coding joints isolated from BOSC 23 cells transfected with E2A or HEB and RAG1 and RAG2 are diverse and resemble those found in T lineage cells. Genomic DNA from transfected cells was amplified with Vδ2 and Dδ3 primers. The resulting fragments were cloned and sequenced. Dashes indicate bases that have been deleted. Underlined bases represent P nucleotide additions. Bases in lowercase lettering could not be matched to the germline DNA sequence.