Figure 5.

Reduced lymphocytes in BM of vcam-1^{flox /} Δ/TIE2Cre⁺ mice. (A) Representative IgD versus IgM profile of B220⁺ cells in the BM of a vcam-1^flox/flox mouse and a vcam-1^flox/Δ/TIE2Cre⁺ mouse showing reduced IgD^loIgM^hi immature B cells and IgD⁺ B cells in the latter. (B and C) Absolute lymphocyte numbers in the BM of age- and sex-matched vcam-1^flox/flox mice (black bars) and vcam-1^flox/Δ/TIE2Cre⁺ mice (white bars), showing normal B220⁺CD43⁺ pro-B cell numbers but reduced numbers of other lymphocyte types. Significant differences between the two groups (n = 4 each) are indicated (Student's t test, *P < 0.02; **P < 0.01; ***P < 0.002).