Figure 5.

Short-term migration of mature B cells into the BM. Flow cytometric analysis of CD45R/B220⁺ gated lymphocytes in BM of control mice and conditional VCAM-1 mutant mice 20 h after injection of mature splenic PKH-26–labeled B cells. The BM B cells are displayed by their IgD surface and PKH-26 expression. Rectangle F^D contains the donor-derived, PKH-26–labeled, recirculating mature B cells; rectangle F represents the fraction F of mature B cells present in the host. 0.06 ± 0.011% of all acquired events were recirculating B220⁺PKH-26⁺IgD⁺ B cells in control mice, while only 0.03 ± 0.01% of such cells were recovered from conditional VCAM-1 mutant BM. In total, 200,000 events were acquired for each dot plot; four control mice and four conditional VCAM-1 mutant mice were analyzed in two independent assays, of which one representative pair of mice is shown.