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. 2001 May 7;193(9):1067–1076. doi: 10.1084/jem.193.9.1067

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© 2001 The Rockefeller University Press

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Detection of membrane lipids (A), structure (B), and mass spectrometry analysis (C) of L-PG. (A) The polar lipids from S. aureus Sa113 wild type, mprF mutant, and mutant complemented with pRBmprF were analyzed by 2d-TLC. The first panel shows the position of the lipids DPG, PG, L-PG, and the putative 2′ L-PG isomer (L-PG2). Two unidentified lipids were negative (P⁻N⁻) or positive (P⁺N⁺) with both phosphate- or amino group–specific reagents, respectively. (B) Structure of L-PG. (C) Part of the FT-ICR mass spectrogram of the TLC spot lacking in the mprF mutant. The molecular masses of singly protonated ions (m/z) are given above the peaks. The major peaks represent L-PG species with two saturated fatty acids (indicated with a dot; compare Table ). The minor peaks represent species with one unsaturated fatty acid (mass difference of −2) or molecules containing one or two ¹³C atoms (mass differences of +1 or +2).

Detection of membrane lipids (A), structure (B), and mass spectrometry analysis (C) of L-PG. (A) The polar lipids from S. aureus Sa113 wild type, mprF mutant, and mutant complemented with pRBmprF were analyzed by 2d-TLC. The first panel shows the position of the lipids DPG, PG, L-PG, and the putative 2′ L-PG isomer (L-PG2). Two unidentified lipids were negative (P⁻N⁻) or positive (P⁺N⁺) with both phosphate- or amino group–specific reagents, respectively. (B) Structure of L-PG. (C) Part of the FT-ICR mass spectrogram of the TLC spot lacking in the mprF mutant. The molecular masses of singly protonated ions (m/z) are given above the peaks. The major peaks represent L-PG species with two saturated fatty acids (indicated with a dot; compare Table ). The minor peaks represent species with one unsaturated fatty acid (mass difference of −2) or molecules containing one or two ¹³C atoms (mass differences of +1 or +2).