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. 2001 Jul 2;194(1):29–44. doi: 10.1084/jem.194.1.29

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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C57BL/6 mice (n = 5 per group) were inoculated with HBSS (untreated), 200 μg mAb 5E6, or 200 μg mAb 1F8 and infected with MCMV 6 h later. 4 d later splenocytes were isolated. NK1.1⁺CD8⁻ cells were costained with mAb 5E6 (Ly49C/I), 1F8 (Ly49C/I/H), anti-Ly49G2, or a mAb isotype control. In this case they were also costained with mAb to CD8 (as part of an experiment examining both T and NK cell functions), so the NK1.1⁺ cells may include both NK and NK/T cells. This figure shows selective depletions of some of the Ly49 subsets due to this mAb treatment. These same mice were examined for viral load, and those data are listed in experiment 1 (Table ).