Figure 3.

Transfection of the mutant 7.19 with mouse MD-2 confers normal sensitivity to LPS. The parental cell line 3E10 was cotransfected with plasmid pcDNA3 and the pELAM-luc reporter plasmid (black bars), as described in the legend to Fig. 2. Similarly, mutant strain 7.19 was cotransfected with mouse MD-2 and pELAM-luc reporter plasmid (hashed bars). Subsequently, cells were stimulated with medium alone, LPS, or IL-1β for 5 h, and cellular lysates were assayed for luciferase activity. The results shown are mean values of triplicate determinations ±SD and are representative of three independent experiments.