Table 2.

Leukocyte Capture and Rolling in Various Situations in the Microcirculation

Vessel type/ treatment	Animal(N/n)	Rolling flux	RLFF	Total capture	TCE/mm2	Primary capture	PCE/mm2	Secondary capture	SCE/mm2
			%		%		%		%
Arterioles
	WT (20/83)	23 ± 1.8	6.3 ± 0.59	6.3 ± 0.63	115 ± 20	4.0 ± 0.36	80 ± 16	2.3 ± 0.31	35 ± 6.6
hIL1β + hTNFα	L−/− (7/34)	7.7 ± 1.5	3.5 ± 1.3	3.4 ± 0.54	55 ± 14	3.3 ± 0.50	54 ± 14	0.13 ± 0.048	1.1 ± 0.45
		P < 0.001	P < 0.001	P = 0.001	P = 0.005	P = 0.118	P = 0.090	P < 0.001	P < 0.001
WT anti-L:	Before (5/34)	24 ± 2.6	7.3 ± 1.0	5.3 ± 0.59	136 ± 28	3.2 ± 0.34	90 ± 21	2.2 ± 0.35	47 ± 10
	After (5/34)	8.3 ± 0.90	5.2 ± 0.72	1.9 ± 0.30	108 ± 33	1.9 ± 0.30	107 ± 33	0.017 ± 0.017	0.70 ± 0.70
		P < 0.001	P < 0.034	P < 0.001	P = 0.505	P < 0.001	P = 0.258	P < 0.001	P < 0.001
Venules
Trauma	WT (23/157)	38 ± 2.5	10 ± 1.1	1.7 ± 0.25	27 ± 5.1	0.98 ± 0.14	16 ± 3.1	0.71 ± 0.11	11 ± 2.3
All ∅	L−/− (7/51)	20 ± 2.0	5.9 ± 1.2	0.85 ± 0.28	20 ± 8.8	0.83 ± 0.27	19 ± 8.7	0.020 ± 0.014	0.37 ± 0.34
		P < 0.001	P < 0.001	P = 0.100	P = 0.074	P = 0.487	P = 0.303	P < 0.001	P < 0.001
WT anti-L:	Before (7/52)	43 ± 3.8	8.6 ± 0.86	1.9 ± 0.32	19 ± 3.6	1.1 ± 0.20	12 ± 2.4	0.81 ± 0.14	7.5 ± 1.3
	After (7/52)	12 ± 1.5	5.0 ± 0.72	0 .45 ± 0.10	16 ± 4.2	0.45 ± 0.099	16 ± 4.2	0.0052 ± 0.0052	0.011 ± 0.011
		P < 0.001	P < 0.001	P < 0.001	P = 0.097	P < 0.001	P = 0.915	P < 0.001	P < 0.001
Trauma	WT (23/71)	30 ± 3.2	14 ± 2.0	1.1 ± 0.33	41 ± 9.9	0.61 ± 0.16	24 ± 6.1	0.50 ± 0.17	17 ± 4.5
∅ < 45 μm	L−/− (7/24)	17 ± 3.0	10 ± 2.2	0.43 ± 0.18	36 ± 18	0.41 ± 0.16	35 ± 18	0.020 ± 0.020	0.73 ± 0.73
		P = 0.033	P = 0.246	P = 0.379	P = 0.294	P = 0.580	P = 0.564	P = 0.021	P = 0.023
WT anti-L:	Before (7/25)	25 ± 3.9	9.1 ± 1.4	0.60 ± 0.20	17 ± 6.3	0.37 ± 0.14	11 ± 4.4	0.23 ± 0.071	6 ± 2.1
	After (7/25)	7.2 ± 1.4	6.9 ± 1.2	0.19 ± 0.075	18 ± 7.4	0.19 ± 0.075	18 ± 7.4	0	0
		P < 0.001	P = 0.219	P = 0.021	P = 0.946	P =0.147	P = 0.622	P = 0.004	P = 0.004
Trauma	WT (23/86)	45 ± 3.6	6.4 ± 0.64	2.2 ± 0.36	15 ± 3.3	1.3 ± 0.23	8.6 ± 1.7	0.88 ± 0.15	6.3 ± 1.7
∅ > 45 μm	L−/− (7/27)	23 ± 2.4	2.0 ± 0.27	1.2 ± 0.51	4.5 ± 1.6	1.2 ± 0.49	4.4 ± 1.5	0.019 ± 0.019	0.052 ± 0.052
		P = 0.009	P < 0.001	P = 0.194	P = 0.057	P = 0.758	P = 0.275	P < 0.001	P < 0.001
WT anti-L:	Before (7/27)	62 ± 4.1	7.7 ± 0.89	3.3 ± 0.49	22 ± 3.9	1.9 ± 0.32	12 ± 2.5	1.4 ± 0.21	9.3 ± 1.7
	After (7/27)	16 ± 2.3	3.3 ± 0.68	0.68 ± 0.17	13 ± 3.6	0.067 ± 0.16	13 ± 3.6	0.010 ± 0.010	0.022 ± 0.022
		P < 0.001	P < 0.001	P < 0.001	P = 0.042	P = 0.002	P = 0.516	P < 0.001	P < 0.001
hIL1β + hTNFα	WT (7/22)	12 ± 3.3	5.6 ± 1.0	0.74 ± 0.34	5.2 ± 2.0	0.48 ± 0.25	3.5 ± 1.5	0.26 ± 0.14	1.7 ± 0.98

All parameters are expressed as mean ± SEM in the indicated number of animals and vessels (N/n). Values of significance (P) are indicated below compared groups in the respective columns. Total capture efficiency (TCE)/mm², primary capture efficiency (PCE)/mm² and secondary capture efficiency (SCE)/mm² represent total-, primary, and secondary capture efficiency adjusted for differences in the observed vessel area, respectively. Anti-L indicates treatment with the L-selectin blocking antibody MEL-14. ∅ indicates vessel diameter.