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. 2001 Jul 16;194(2):219–226. doi: 10.1084/jem.194.2.219

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The migration of neutrophils at 4 h after TG treatment into the peritoneum of LFA-1^+/+ and ^−/− mice in the presence of mAbs specific for LFA-1, Mac-1, and α4 integrins either singly or in combination. For LFA-1^+/+ mice, anti–LFA-1 and anti–Mac-1 mAbs, when administered alone, significantly inhibit neutrophil migration. All mAb pairs were significantly effective in inhibiting migration compared with the control. In addition, all pairs of mAbs block significantly more than single mAbs (P < 0.001), indicating that α4 integrin cooperates with LFA-1 and Mac-1 in the migration of LFA-1^+/+ neutrophils. For LFA-1^−/− mice, only anti-α4 integrin inhibits neutrophil migration as a single mAb and does not inhibit more significantly when in combination with anti–Mac-1 mAb (α4 versus α4/Mac-1 mAbs; P > 0.05). These data are representative of three complete experiments which utilized 3–10 mice per condition per experiment. Data are reported as mean ± SEM.

The migration of neutrophils at 4 h after TG treatment into the peritoneum of LFA-1^+/+ and ^−/− mice in the presence of mAbs specific for LFA-1, Mac-1, and α4 integrins either singly or in combination. For LFA-1^+/+ mice, anti–LFA-1 and anti–Mac-1 mAbs, when administered alone, significantly inhibit neutrophil migration. All mAb pairs were significantly effective in inhibiting migration compared with the control. In addition, all pairs of mAbs block significantly more than single mAbs (P < 0.001), indicating that α4 integrin cooperates with LFA-1 and Mac-1 in the migration of LFA-1^+/+ neutrophils. For LFA-1^−/− mice, only anti-α4 integrin inhibits neutrophil migration as a single mAb and does not inhibit more significantly when in combination with anti–Mac-1 mAb (α4 versus α4/Mac-1 mAbs; P > 0.05). These data are representative of three complete experiments which utilized 3–10 mice per condition per experiment. Data are reported as mean ± SEM.