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. 2001 Jul 16;194(2):219–226. doi: 10.1084/jem.194.2.219

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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The effect of LFA-1 deficiency on neutrophil rolling, adhesion, and transmigration as detected by intravital microscopy. A comparison in LFA-1^+/+ and ^−/− mice of leukocyte (A) velocity (V_WBC); (B) integrated cell flux (i.e., cell flux per 10⁶ leukocytes per milliliter of blood). Inset, basic cell flux (cells per min); (C) cell adhesion; (D) emigrated leukocytes per field (“field” represents cells within 50-μm distance from a 100-μm segment of vessel). The neutrophils from LFA-1^−/− mice have a higher velocity than those from LFA-1^+/+ mice indicating a role for LFA-1 in neutrophil rolling. Because LFA-1 does not alter the cell flux (frequency of contact with endothelium), it must be decreasing the velocity by transiently stabilizing the rolling cells. A lack of LFA-1 is also reflected in lower adhesion per 100-μm length of mesenteric vessel and decreased emigration from the vasculature by the LFA-1^−/− as compared with LFA-1^+/+ neutrophils. Data are reported as mean ± SEM.

The effect of LFA-1 deficiency on neutrophil rolling, adhesion, and transmigration as detected by intravital microscopy. A comparison in LFA-1^+/+ and ^−/− mice of leukocyte (A) velocity (V_WBC); (B) integrated cell flux (i.e., cell flux per 10⁶ leukocytes per milliliter of blood). Inset, basic cell flux (cells per min); (C) cell adhesion; (D) emigrated leukocytes per field (“field” represents cells within 50-μm distance from a 100-μm segment of vessel). The neutrophils from LFA-1^−/− mice have a higher velocity than those from LFA-1^+/+ mice indicating a role for LFA-1 in neutrophil rolling. Because LFA-1 does not alter the cell flux (frequency of contact with endothelium), it must be decreasing the velocity by transiently stabilizing the rolling cells. A lack of LFA-1 is also reflected in lower adhesion per 100-μm length of mesenteric vessel and decreased emigration from the vasculature by the LFA-1^−/− as compared with LFA-1^+/+ neutrophils. Data are reported as mean ± SEM.