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. 2001 Jul 16;194(2):143–154. doi: 10.1084/jem.194.2.143

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Stat6 is required for IL-4–mediated downregulation of IL-18Rα expression and IL-18–induced IFN-γ. CD4⁺ T cells from wild-type BALB/c (A–C and G–K) and Stat6^−/− (D–F and G–K) mice were stimulated for two d with plate-bound anti-CD3 and IL-2 alone (A and D), or with IL-2 and IL-4 (B and E) or IL-12 (C and F). The cells were then washed and placed into fresh IL-2 media with fresh cytokine for an additional 2 d. Cells were then stained for IL-18Rα expression. Alternatively, cells from BALB/c or Stat6^−/− mice were primed for 7 d with anti-CD3 and IL-2, then restimulated with either anti-CD3 (G), IL-2 (H), IL-12 (I and K), or IL-18 (J and K). Cells were stained for intracellular IFN-γ and IL-4.

Stat6 is required for IL-4–mediated downregulation of IL-18Rα expression and IL-18–induced IFN-γ. CD4⁺ T cells from wild-type BALB/c (A–C and G–K) and Stat6^−/− (D–F and G–K) mice were stimulated for two d with plate-bound anti-CD3 and IL-2 alone (A and D), or with IL-2 and IL-4 (B and E) or IL-12 (C and F). The cells were then washed and placed into fresh IL-2 media with fresh cytokine for an additional 2 d. Cells were then stained for IL-18Rα expression. Alternatively, cells from BALB/c or Stat6^−/− mice were primed for 7 d with anti-CD3 and IL-2, then restimulated with either anti-CD3 (G), IL-2 (H), IL-12 (I and K), or IL-18 (J and K). Cells were stained for intracellular IFN-γ and IL-4.