Table 2.
Clinical Assessment of Joint Stiffness in gp130ΔSTAT/STAT Mice
| Age (d) | 30 | 50 | 71 | 98 | 141 | 176 | |
|---|---|---|---|---|---|---|---|
| Limb | |||||||
| Mouse 1 | Rear left | 1 | 1 | 1 | 1 | 2 | 2 |
| Rear right | 1 | 1 | 1 | 1 | 1 | 1 | |
| Front left | 0 | 0 | 0 | 0 | 0 | 0 | |
| Front right | 2 | 2 | 2 | 2 | 2 | 2 | |
| Mouse 2 | Rear left | 1 | 1 | 1 | 1 | 2 | 2 |
| Rear right | 1 | 1 | 1 | 1 | 1 | 1 | |
| Front left | 0 | 0 | 0 | 1 | 1 | 1 | |
| Front right | 0 | 0 | 0 | 1 | 1 | 1 | |
| Mouse 3 | Rear left | 1 | 1 | 0 | 1 | 1 | 2 |
| Rear right | 1 | 1 | 1 | 2 | 1 | 1 | |
| Front left | 0 | 0 | 0 | 0 | 0 | 0 | |
| Front right | 0 | 0 | 0 | 0 | 0 | 0 | |
| Average score per gp130ΔSTAT/STAT mouse (n = 14) | 3.3 ± 1.2 | 2.9 ± 1.7 | 3.0 ± 1.2 | 3.3 ± 1.2 | 3.5 ± 1 | 3.5 ± 1.1 | |
| Average score per gp130ΔSTAT/wt mouse (n = 21) | 0 | 0 | 0 | 0 | 0 | 0 |
Mice were clinically assessed and scores for joint movement were allocated as described in Materials and Methods. Each limb of mice in cohorts of gp130ΔSTAT/ΔSTAT and gp130ΔSTAT/wt animals was assessed twice per week for restriction in wrist and ankle movement as illustrated in Fig. 4 c to f. Scores were allocated for: 0, no restriction; 1, moderate restriction; and 2, severe restriction, with 8 being the maximal score per mouse. None of the gp130ΔSTAT/wt control mice scored positive at any time point. The table shows the results for selected time points of three representative gp130ΔSTAT/ΔSTAT mice and the average score per mouse in the mutant cohort (mean ± SD, n = 14).