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. 2001 Aug 6;194(3):365–374. doi: 10.1084/jem.194.3.365

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Acetylation states of H3 associated with tet promoter in SCT(μ,α) transfectant clone #3 before and after transcription induction. Soluble chromatins were prepared from SCT(μ,α) transfectant clone #3 cultured for 24 h in the presence or absence of S region transcription and switch stimulation. Antibodies against acetylated histone H3 (αAcH3) or rabbit IgG (Control) were used to isolate DNAs associated with the specific antigens. Fivefold serial dilutions of purified DNA were amplified by semiquantitative PCR with primers for tet, CD3ε, CD19, or Iα promoters. Total DNA before immunoprecipitation (IP) was used as an internal control of PCR. PCR cycles for CD3ε and the rest were 40 and 30, respectively.