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. 2001 Aug 6;194(3):255–264. doi: 10.1084/jem.194.3.255

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Loss of the LMP2A developmental and survival phenotype when bred into the SLP-65^−/− background. (A) Mouse splenocytes (SP) and (B) bone marrow samples (BM) from WT, TgE SLP-65^−/−, and TgE SLP-65^−/− animals were subjected to flow cytometric analysis with at least 10,000 gated lymphocytes collected per animal using CD19-PE (CD19) and IgM-FITC (IgM) antibodies. Typical dot plot results of 10,000 gated lymphocytes from 5–7-wk-old animals (n = 5) are shown. The polygons represent distinct subpopulations of B cells with the average percentage of total gated lymphocytes indicated above each polygon. (A) The left polygon represents CD19⁺IgM⁻ B cells typical in TgE animals; the right polygon represents CD19⁺IgM⁺ cells. (B) The left rectangle represents CD19⁺IgM⁻ B cells; the right rectangle represents CD19⁺IgM⁺ B cells.