Figure 3.

Expression of wt ZAP-70 regulated by the lck proximal promoter reconstitutes both positive and negative selection of H-Y TCR⁺ thymocytes. (A) H-Y TCR⁺ thymocytes (top) and splenocytes (bottom) from zap-70 ^+/−, wt ZAP-70⁺ zap-70⁻/−, or zap-70 ^−/ − H-Y TCR⁺ female mice were analyzed for CD4 and CD8 expression by FACS^® analysis. The number of thymocytes isolated from each mouse is depicted above each analysis. Thymocytes shown represent the H-Y TCR⁺ population as defined by T3.70 staining. Average thymocyte number isolated for each genotype is represented in Table . (B) H-Y TCR⁺ thymocytes (top) and splenocytes (bottom) from zap-70 ^+/−, wt ZAP-70⁺ zap-70⁻/−, or zap-70 ^−/ − H-Y TCR⁺ male mice were analyzed by FACS^® analysis as described above. Thymocytes shown represent the H-Y TCR⁺ population as defined by T3.70 staining. Average thymocyte number isolated for each genotype is represented in Table .