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. 2001 Aug 20;194(4):471–480. doi: 10.1084/jem.194.4.471

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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RAG1 is expressed in DN T cells in RYAC transgenic mice. (A) Diagrammatic representation of RYAC an 80-kb YAC containing the RAG genes, 12 kb of genomic DNA 5′ of RAG2, and 33 kb of genomic DNA 5′ of RAG1. (B) RAG1 expression in FACS^® purified thymocyte subpopulations as measured by RT-PCR. HPRT, CD3ε, or GAPDH were used as loading controls for normalizing mRNA. The data shown are representative of five experiments. cDNA dilutions are indicated. WT, wild-type.

RAG1 is expressed in DN T cells in RYAC transgenic mice. (A) Diagrammatic representation of RYAC an 80-kb YAC containing the RAG genes, 12 kb of genomic DNA 5′ of RAG2, and 33 kb of genomic DNA 5′ of RAG1. (B) RAG1 expression in FACS^® purified thymocyte subpopulations as measured by RT-PCR. HPRT, CD3ε, or GAPDH were used as loading controls for normalizing mRNA. The data shown are representative of five experiments. cDNA dilutions are indicated. WT, wild-type.