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. 2001 Aug 20;194(4):471–480. doi: 10.1084/jem.194.4.471

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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5′ skewed Jα usage in RYIIRAG1 ^−/− and RYIIRAG2 ^−/− mice. (A) Percentage of TCRα mRNAs using Jαs found within the indicated 10-kb intervals in the Jα locus. The results represent 68 RYIIRAG1 ^−/− and 24 wild-type (WT) clones from the thymus, and 33 RYIIRAG1 ^−/− and 36 WT clones from the spleen. Schematic representation of the Jα locus is shown and position of Jαs from GenBank/EMBL/DDBJ accession no. M64239 with distances in kbs, the position of the PCR primers and probes used in B is shown. (B) V-Jα rearrangement measured by PCR on genomic DNA from total thymus. RAG1 ^−/− mouse tail DNA was used as a negative control. The first lane in each case represents ∼60 ng of template DNA diluted as shown. PCR on CD14 was used as loading control. Results are representative of two separate experiments with individual mouse samples.

5′ skewed Jα usage in RYIIRAG1 ^−/− and RYIIRAG2 ^−/− mice. (A) Percentage of TCRα mRNAs using Jαs found within the indicated 10-kb intervals in the Jα locus. The results represent 68 RYIIRAG1 ^−/− and 24 wild-type (WT) clones from the thymus, and 33 RYIIRAG1 ^−/− and 36 WT clones from the spleen. Schematic representation of the Jα locus is shown and position of Jαs from GenBank/EMBL/DDBJ accession no. M64239 with distances in kbs, the position of the PCR primers and probes used in B is shown. (B) V-Jα rearrangement measured by PCR on genomic DNA from total thymus. RAG1 ^−/− mouse tail DNA was used as a negative control. The first lane in each case represents ∼60 ng of template DNA diluted as shown. PCR on CD14 was used as loading control. Results are representative of two separate experiments with individual mouse samples.