Figure 2.

K14-A_β ^b CD4⁺25⁺ cells are anergic and suppressive. (A) CD4⁺25⁺ cells (5 × 10⁴) from K14-A_β ^b or B6 (WT) were cultured with MHC class II-negative APCs (10⁵), soluble anti-CD3ε (1 μg/ml), and rIL-2 (10 U/ml) for 72 h, pulsed with [³H]thymidine for 16 h and harvested. (B) CD4⁺25⁻ cells (5 × 10⁴) from K14-A_β ^b or B6 mice were incubated with MHC class II-negative APCs (10⁵), soluble anti-CD3ε (1 μg/ml), and the indicated number of CD4⁺25⁺ cells from K14-A_β ^b or B6 mice for 72 h, pulsed with [³H]thymidine for the final 16 h and harvested. Results are representative of three experiments.