FIG. 6.

HIS7 mRNA levels in yeast cells expressing either a low or a high amount of Gcn4 protein after cultivation without exogenous adenine. Deletions of components required for chromatin modification are as indicated. Low Gcn4p amounts are expressed from the wild-type GCN4 gene (−), whereas high Gcn4p amounts are expressed from plasmid p238 (+) for strains FY1353 (wt), FY1360 (snf2D), FY1354 (gcn5D), and FY1352 (snf2Δ/gcn5D). Four independent RNA isolations were hybridized twice in Northern experiments and then equalized to ACT1 mRNA levels, resulting in the mean values given in the graph.