TABLE 1.

Yeast strains and plasmids used in this study

Strain or plasmid	Genotype or descriptiona	Reference or source
Strains
FY1353	MATα his3Δ200 ura3-52 leu2Δ1 lys2-173R2	39
RH2561	Same as FY1353 but with plasmid pRS303 (HIS3)	This work
FY1354	MATα his3Δ200 ura3-52 leu2Δ1 lys2-173R2 gcn5Δ::HIS3	39
FY1360	MATa his3Δ200 ura3-52 leu2Δ1 lys2-173R2 snf2Δ::LEU2	39
RH2563	Same as FY1360 but with plasmid pRS303 (HIS3)	This work
FY1352	MATa his3Δ200 ura3-52 leu2Δ1 lys2-173R2 gcn5Δ::HIS3 snf2Δ::LEU2	39
RH1381	MATa ura3-52 aro3-2 gcn4-101	37
RH1616	MATa ura3-52 aro3-2 his7-lacZ gcn4-101	37
RH2029	MATa ura3-52, gcn4-101 bas1-2 bas2-2	45
RH2569	MATa ura3-52 aro3-2 his7-lacZ gcn5Δ::loxP	This work
RH2570	MATa ura3-52 aro3-2 his7-lacZ gcn4-101 gcn5Δ::loxP	This work
RH1619	MATa ura3-52 aro3-2 his7(mut GCRE1)-lacZ gcn4-101	37
RH1622	MATa ura3-52 aro3-2 his7(mut GCRE2)-lacZ gcn4-101	37
RH1830	MATa ura3-52 aro3-2 his7(mut ABS)-lacZ gcn4-101	36
Plasmids
pBluescript II SK(+)	Commercial cloning vector with polylinker; lacZ	Stratagene
pUG6	loxP-kanMX-loxP module with Kanr marker flanked by TEF2 promoter and terminator, Ampr	17
pME1768	loxP-kanMX-loxP module from pUG6 in pBluescript II SK(+)
pME2034	774-bp GCN5 3′ fragment in pBluescript II SK(+)	This work
pME2035	862-bp GCN5 5′ fragment in pBluescript II SK(+)	This work
pME2036	1.6-kb kanMX fragment from pME1768 in between GCN5 5′ and 3′ fragment	This work
pSH47	cre recombinase expression vector	17
pRS303	Yeast integrative vector; lacZ HIS3	35
pCB159	pGAL-BAS1 (SpeI-MluI 3.6-kb fragment) in B656 backbone	44
p238	YCp50 carrying a GCN4 allele with mutated uORFsb	27

^aKan^r, kanamycin resistance; Amp^r, ampicillin resistance.

^buORFs, upstream open reading frames.