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. 2001 Aug 20;194(4):393–406. doi: 10.1084/jem.194.4.393

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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IFN-γ–induced activation of Mhc2ta expression is mediated largely by pI. Thioglycollate-elicited peritoneal macrophages from control (+/−) or homozygous mutant (−/−) mice were cultured in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of IFN-γ. Expression of CIITA was analyzed by RNase protection using probes specific for type IV (A), type I (B), and type III (C) mRNA. The band corresponding to the specific mRNA (I, III, or IV) and the band representing mRNA derived from the other two promoters (NonI, NonIII, and NonIV) are indicated. A probe for GAPDH mRNA was used as internal control. The percentages of total CIITA mRNA corresponding to types I, III, and IV were quantified by PhosphorImager analysis of RNase protection experiments similar to those shown in A–C (D).

IFN-γ–induced activation of Mhc2ta expression is mediated largely by pI. Thioglycollate-elicited peritoneal macrophages from control (+/−) or homozygous mutant (−/−) mice were cultured in the absence (lanes 1 and 3) or presence (lanes 2 and 4) of IFN-γ. Expression of CIITA was analyzed by RNase protection using probes specific for type IV (A), type I (B), and type III (C) mRNA. The band corresponding to the specific mRNA (I, III, or IV) and the band representing mRNA derived from the other two promoters (NonI, NonIII, and NonIV) are indicated. A probe for GAPDH mRNA was used as internal control. The percentages of total CIITA mRNA corresponding to types I, III, and IV were quantified by PhosphorImager analysis of RNase protection experiments similar to those shown in A–C (D).