Table 1.
Specificity of HEL48–61 Reactive T Cells
| HEL48–61 | ||||
|---|---|---|---|---|
| N59 | D59 | βD59 | ||
| Type A | CP4.1 | ++ | ++ | ++ |
| CP4.22 | +++ | +/− | ++ | |
| 3A9 | +++ | − | − | |
| ALV11 | ++ | − | − | |
| Type B | ALV48 | ++ | +++ | + |
| DAV21 | ++ | +++ | +/− | |
| CP3.43 | +++ | +++ | ++ | |
| CP3.42 | +++ | +++ | ++ | |
| CP3.12 | ++ | ++ | + | |
| B10A8.4 | +++ | +/− | +++ | |
| CP1.9 | +++ | + | + | |
| CP1.7 | +++ | +++ | + | |
| CP1.17 | +++ | +++ | ++ | |
| CP3.11 | +++ | ++ | − | |
| CP4.17 | ++ | + | + | |
| MLA11.2 | +++ | − | +/− | |
| B61.6 | +++ | − | − | |
| MLC10.22 | ++ | − | − | |
| CP2.11 | ++ | − | − | |
| B10A6.5 | +++ | − | − | |
| Isoaspartyl | Iso.187 | − | − | ++ |
| Iso.210 | − | − | ++ | |
| Iso.198 | − | + | ++ | |
| Iso.203 | +++ | + | +++ | |
| Iso.156 | +++ | − | +++ | |
Patterns of reactivities to peptides among type A and B T cells. Type A T cell hybridomas recognize native HEL and HEL48–61 N59 with similar sensitivity, while type B hybridomas are at least 100-fold more sensitive to peptide than to native HEL. Shown here is a list of T cell hybridomas that follow the criteria for A and B T cells. References 3 and 4 have reported on the reactivity of many of these. The “Iso” series of hybridomas were generated by immunization with HEL48–61βD59. All hybridomas were tested against the following synthetic peptides: HEL48–61 N59 (indicated as N59), HEL48–61D59 (D59), or HEL48–61βD59 (βD59). For easier comparison, the sensitivity of the hybridomas to the given peptides are represented as: +++ for 0.1 μM or less, ++ for 1–10 μM, + for greater than 10 μM, +/− for trace response, − for no response at the highest concentration tested.