Table 2.
Examples of Genes Regulated by Viable Mtb, Heat-killed Mtb, and Polystyrene Particles
| Type of response | Gene product | Impact of IFN-γ (fold change) | Impact of particle(fold change) |
|---|---|---|---|
| Induction only by viable Mtb | MHC class III complement factor B | NA | 11 |
| IFN-induced Mx protein | 9.4 | ||
| Intercrine | 7.7 | ||
| IFN regulatory factor 7 (mirf7) | 6.9 | ||
| JE chemokine | 6.2 | ||
| IP-10 chemokine | 5.7 | ||
| IGTP | 4.0 | ||
| JAK-binding protein (JAB, SOCS1) | 3.5 | ||
| Induction only by heat-killed Mtb | KC chemokine | 96 | |
| ASS | 14 | ||
| Integrin α5 subunit | 13 | ||
| gp91component of phox | 5.1 | ||
| C/EBPβ | 4.5 | ||
| TNF | 4.1 | ||
| HIF-1α | 3.7 | ||
| IL-2 receptor γ | 2.6 | ||
| Antioxidant protein 2 (peroxiredoxin) | 2.5 | ||
| Induction only by polystyrene beads | Annexin III (lipocortin III) | 8.1 | |
| Induction by all three particles | MIP2 chemokine | 43 | |
| Suppression only by heat-killed Mtb | MCP-1 (chemokine) receptor | 0.24 | |
| IFN-α1 | 0.45 | ||
| Suppression only by polystyrene beads | Irg-47 | 0.28 | |
| MAP kinase (ERK-1) | 0.47 | ||
| Suppression by all three particles | CTLA-2β | 0.26 | |
| Induction by the combination of IFN-γ only with viable Mtb | CD44 | 3.7 | 11 |
| Fractalkine (chemokine) | ns | 3.9 | |
| Induction by the combination of IFN-γ only with heat-killed Mtb | IL-12 p40 | ns | 16 |
| HIF-1α | ns | 6.1 | |
| FcγRIIB | ns | 4.0 | |
| Induction by the combination of IFN-γ with any of the particles | MIG (chemokine) | 223 | 670 |
| RANTES | 137 | 578 | |
| NOS2 | 28 | 568 | |
| Prostaglandin synthase | 6.9 | 172 | |
| IP-10 chemokine | 32 | 83 | |
| CD1.1 | 6.6 | 28 | |
| CD40 | 5.6 | 21 | |
| C-C CKR-1 | 3.6 | 18 | |
| Suppression by the combination of IFN-γ only with viable Mtb | Scavenger receptor class B type I | ns | 0.12 |
| Suppression by the combination of IFN-γ only with heat-killed Mtb | Plasma glutathione peroxidase | 0.48 | 0.06 |
CKR, chemokine receptor; HIF, hypoxia-inducible factor; MAP, monocyte activating peptide; MCP, monocyte chemoattractant protein; NA, not applicable; ns, regulation not statistically significant.