Table 4.
Examples of the Impact of Deficiency of iNOS and Phox on Regulation of Gene Expression by IFN-γ and Mtb
| Knockout | Stimulus | iNOS and/or phox help mediate induction or resist suppression by IFN-γ and/or Mtb | iNOS and/or phox antagonize induction by IFN-γ and/or Mtb | ||
|---|---|---|---|---|---|
| Gene | Relative impact | Gene | Relative impact | ||
| iNOS | IFN-γ | ASS | 0.08 | MHC class I Qa | 40 |
| IFN-β | 0.18 | EMAP | 16 | ||
| TIMP-3 | 0.18 | p68 lipocortin | 2.9 | ||
| DAF | 2.6 | ||||
| G-CSF | 2.2 | ||||
| IGTP | 2.0 | ||||
| Mtb | MHC class I Qa | 62 | |||
| PIRB1 | 2.1 | ||||
| IFN-γ plus Mtb | CD53 ho | 0.13 | MHC class I Qa | 46 | |
| RANTES | 0.16 | p68 lipocortin | 4.4 | ||
| PC4 ho | 0.21 | IAP | 2.4 | ||
| Integrin α4 | 2.3 | ||||
| phox | IFN-γ | ASS | 0.13 | ||
| Mtb | IFI 204 ho | 1.8 | |||
| IFN-γ plus Mtb | ASS | 0.16 | Leu 13 (9–27) ho | 4.2 | |
| JAK2 | 0.43 | PIRAI | 3.0 | ||
| MMP1 | 0.46 | CD97 prec ho | 2.6 | ||
| MCP-5 prec | 1.7 | ||||
| IL-15 | 1.6 | ||||
| LRG-47 | 1.6 | ||||
| iNOX plus phox | IFN-γ | ASS | 0.04 | Mannose R | 2.4 |
| IκB-β | 0.45 | SHP | 2.3 | ||
| Irg-1 | 0.46 | ||||
| Mtb | JAK3 | 4.1 | |||
| PIRB1 | 2.8 | ||||
| TGF-β3 | 2.8 | ||||
| PIRA6 | 2.6 | ||||
| IFI 204 ho | 2.5 | ||||
| Galectin 3 | 2.3 | ||||
| IFN-γ plus Mtb | MRP-8 | 0.13 | Integrin α6 | 11 | |
| ASS | 0.13 | Leu 13 (9–27) ho | 4.0 | ||
| H2-M | 3.3 | ||||
| p68 lipocortin | 2.6 | ||||
| MCP-5 prec | 2.4 | ||||
ASS, argininosuccinate synthetase; CD97, leukocyte marker and DAF-binding protein; DAF, decay accelerating factor (complement regulator; CD55); EMAP, endothelial monocyte activating peptide; H2-M, MHC class II-like molecule that facilitates peptide loading onto class II; IAP, integrin-associated protein (CD47); IFI, IFN-induced nuclear phosphoprotein; IκB, inhibitor of NF-κβ; Leu 13, IFN-inducible leukocyte and endothelial protein involved in adhesion; LRG-47, large G protein-related protein of 47 kDa; MMP, matrix metalloproteinase; PC4, immediate early gene induced by IFN-γ in various cells; SHP, src homology domain-containing tyrosine phosphatase; TIMP, tissue inhibitor of metalloproteinases.