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. 2003 Oct;2(5):1009–1017. doi: 10.1128/EC.2.5.1009-1017.2003

FIG. 3.

FIG. 3.

The 3′ UTR is required for regulation of PFR2 mRNAs. (A) Schematic representation of PFR2 DNA cassettes. Symbols are as described in the legend to Fig. 2A. (B) Northern analysis of total RNA from promastigotes (P) and amastigotes (A) of wild-type L. mexicana (WT) and the Δpfr2 strain transfected with the indicated plasmids probed with PFR2 coding sequence. Numbers below the autoradiogram are ratios of PFR2 mRNA in promastigotes to that in amastigotes (P/A), determined as described in the legend to Fig. 2B.