FIG. 4.

Deletion analysis of the PFR2C 3′ UTR. (A) Schematic representation of PFR2C cassettes. Symbols are as described in the legend to Fig. 2A. The position of the deletions is indicated by the absence of a solid bar. (B) Northern analysis of total RNA from promastigotes (P) and amastigotes (A) of the wild type (WT) and of the Δpfr2 strain containing the indicated plasmids probed with PFR2 coding sequence. Ethidium bromide-stained rRNA is shown below the autoradiogram. Numbers below the rRNA are ratios of PFR2 mRNA in promastigotes to that in amastigotes (P/A), determined as described in the legend to Fig. 2B.