FIG. 5.

Identification of regulatory sequences in region 3 of the 3′ UTR. (A) Schematic diagram of further deletions (Δ3.1, Δ3.2, Δ3.3, and Δ3.4) in region 3 of the PFR2C 3′ UTR. The position of the deletions is indicated by the absence of a solid bar. (B) Northern analysis of total RNA from promastigotes (P) and amastigotes (A) of the wild type (WT) and the Δpfr2 line containing the indicated plasmids (p2CΔ3, p2CΔ3.1, p2CΔ3.2., p2CΔ3.3, and p2CΔ3.4) probed with PFR2 coding sequence. Ethidium bromide-stained rRNA is shown below the autoradiogram. Numbers below the rRNA are ratios of PFR2 mRNA in promastigotes to that in amastigotes (P/A), determined as described in the legend to Fig. 2B.