Table 1.
TREM-2–dependent Regulation of Cell Surface Activation Markers
| F(ab′)2 anti–TREM-1 | F(ab′)2 anti–TREM-2 | LPS | |
|---|---|---|---|
| TREM-2 | 112.23 | 3.45 | 7.1 |
| MHC class I | 67.8 | 65.3 | 107.1 |
| MHC class II | 119.12 | 278.65 | 454.67 |
| CD40 | 171.35 | 598.6 | 635.89 |
| CD80 | 32.1 | 39.4 | 104.6 |
| CD86/B7.2 | 14.04 | 387.91 | 683.56 |
| CCR5 | 12.95 | 13.56 | 3.12 |
| CCR6 | 3.68 | 3.45 | 4.01 |
| CCR7 | 6.82 | 21.98 | 12.45 |
| CXCR4 | 5.13 | 4.56 | 17.8 |
| CD11a | 10.92 | 6.78 | 13.72 |
| CD11b | 53.9 | 65.7 | 23.1 |
| CD11c | 91.1 | 65.7 | 123.5 |
| CD29 | 38.22 | 37.56 | 37.5 |
| CD41 | 4.54 | 4.67 | 4.39 |
| CD54/ICAM-1 | 56.87 | 54.78 | 271.45 |
| CD61 | 4.95 | 5.03 | 4.21 |
| CD103 | 3.63 | 3.96 | 3.26 |
| Mannose-R | 81.8 | 82.9 | 30.9 |
| CD64/FcγR I | 9.8 | 10.1 | 2.3 |
| CD32/FcγR II | 17.21 | 16.78 | 2.34 |
| CD89/FcαR | 4.54 | 4.75 | 4.96 |
| CD35/CR 1 | 3.94 | 4.23 | 3.67 |
| M-CSF-R | 14.6 | 4.23 | 5.21 |
| GM-CSF-R | 15.6 | 13.7 | 13.5 |
| CD38 | 2.5 | 2.2 | 43.5 |
| CD83 | 3.34 | 3.23 | 26.7 |
| CD1a | 106.76 | 134.9 | 87.54 |
DCs were cultured for 48 h in plates coated with control F(ab′)2, F(ab′)2 anti–TREM-2 mAb, or LPS as indicated in Fig. 6. Cells were subsequently analyzed by flow cytometry for the indicated cell surface molecules. Numerical values indicate specific mean fluorescence intensity after subtraction of the fluorescence detected with an isotype-matched control. The data shown are representative of four independent experiments.