Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2001 Oct 15;194(8):1033–1042. doi: 10.1084/jem.194.8.1033

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

3-155 antigen is an MR and macrophage and lymphatic MR have identical cell distribution and indistinguishable glycosylation profiles. (A) 3-155 antibody coupled to Sepharose 4B beads via rabbit anti–mouse IgG as well as the positive control precipitation with the commercial anti-MR antibody (same as the detecting antibody) were able to remove MR from lymphocyte lysate when tested by immunoblotting using a commercial anti-MR antibody. After the negative control precipitation with beads coupled to an irrelevant antibody (3G6) the MR band is clearly visible. (B) Histograms from flow cytometric analyses demonstrate that GM-CSF–activated monocytes show positive and identical staining both with a known anti–human MR antibody (α-hMR) and 3-155. 3G6 is a negative control antibody. (C) After various glycosidase treatments of activated monocyte (macrophages) and lymph node lysates (lymphatic), molecules were separated by SDS-PAGE and analyzed by immunoblotting with 3-155 and a negative control antibody (3G6).

3-155 antigen is an MR and macrophage and lymphatic MR have identical cell distribution and indistinguishable glycosylation profiles. (A) 3-155 antibody coupled to Sepharose 4B beads via rabbit anti–mouse IgG as well as the positive control precipitation with the commercial anti-MR antibody (same as the detecting antibody) were able to remove MR from lymphocyte lysate when tested by immunoblotting using a commercial anti-MR antibody. After the negative control precipitation with beads coupled to an irrelevant antibody (3G6) the MR band is clearly visible. (B) Histograms from flow cytometric analyses demonstrate that GM-CSF–activated monocytes show positive and identical staining both with a known anti–human MR antibody (α-hMR) and 3-155. 3G6 is a negative control antibody. (C) After various glycosidase treatments of activated monocyte (macrophages) and lymph node lysates (lymphatic), molecules were separated by SDS-PAGE and analyzed by immunoblotting with 3-155 and a negative control antibody (3G6).