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. 2001 Oct 15;194(8):1043–1052. doi: 10.1084/jem.194.8.1043

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© 2001 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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IL-2 release by T cell hybridomas transfected with the wt 2C or high-affinity mutant m6 TCRs. 2C TCR (○) or m6 TCR (•) transfectants were incubated with T2-L^d APCs in the presence of various concentrations of peptides. After 30 h, supernatants were harvested and assayed for IL-2 by ELISA. Assays were done in triplicate and the mean A₄₅₀ value for each concentration is shown. ELISA curves from one representative experiment are shown for each peptide and transfectant. IL-2 standard curves demonstrated that an A₄₅₀ value of 0.3 corresponded to ∼1,200 pg/ml IL-2. The null peptide MCMV did not elicit detectable IL-2 at even the highest concentration tested (1 μM).