Figure 5.

The effects of ionomycin and compound 48/80 on mast cell A1 expression, mediator release, and survival. RPA was performed on RNA derived from MCP5/L cells incubated for 6 h in the presence of ionomycin or compound 48/80 at concentrations indicated (A and D). Release of β-hexosaminidase (β-hex) after 30 min was measured using an enzymatic colorimetric method and was indicative of activation and degranulation of mast cells (B and E). Survival rate of mast cells after 4 days in medium deprived of serum and growth factors was measured (C and F) as explained in Fig. 1. Data shown in A and D are a representative of three separate experiments and data shown in the rest are expressed as the mean ± SEM from three experiments.