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. 2002 Jun 3;195(11):1397–1406. doi: 10.1084/jem.20020141

Figure 2.

Figure 2.

Screening of an Ii-cDNA library from RNA of F27mel using CD4+ F27TIL-T1 cells. (A) Identification of positive cDNA clones encoding a tumor antigen recognized by CD4+ T cells. After the screening of 2 × 105 Ii-cDNA fusion library clones generated from F27mel RNA, positive cDNA pools were identified on the basis of GM-CSF release from CD4+ F27TIL-T1. CD4+ F27TIL-T1 recognized 293IMDR2 transfected with the cDNA clone 3, but not with a control cDNA or green fluorescent protein. (B) Schematic presentation of FN protein and the position of the mutated amino acid.