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. 2002 Jun 3;195(11):1397–1406. doi: 10.1084/jem.20020141

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Loss of FN matrix formation in F27mel cells harboring a mutated FN. (A) Immunostaining of FN matrix of three melanoma cell lines (1143mel, 1195mel, and F27mel) with Ab-9 anti-FN Ab. FN was detected by indirect immunofluorescence. Phase contrast and fluorescence images were taken at ×40. (B) Genomic DNA sequence analysis of FN in three melanoma cell lines. Genomic DNA fragments were amplified by FN-specific primers. The PCR products were sequenced to identify mutated FN. (C) Comparison of immunostaining for FN matrix in 1143mel and F27mel cells with different anti-FN (Ab-1, Ab-7, AB-8, and Ab-9) Abs that recognize epitopes in the different regions of FN. DAPI staining was used as controls for cell density. Staining of FN matrix in 1143mel and F27mel cells with all four Abs showed these similar patterns: intensive staining of FN in 1143mel cells, but little or weak staining in F27mel cells. 1143 and F27 stand for 1143mel and F27mel, respectively.

Figure 4. — Loss of FN matrix formation in F27mel cells harboring a mutated FN. (A) Immunostaining of FN matrix of three melanoma cell lines (1143mel, 1195mel, and F27mel) with Ab-9 anti-FN Ab. FN was detected by indirect immunofluorescence. Phase contrast and fluorescence images were taken at ×40. (B) Genomic DNA sequence analysis of FN in three melanoma cell lines. Genomic DNA fragments were amplified by FN-specific primers. The PCR products were sequenced to identify mutated FN. (C) Comparison of immunostaining for FN matrix in 1143mel and F27mel cells with different anti-FN (Ab-1, Ab-7, AB-8, and Ab-9) Abs that recognize epitopes in the different regions of FN. DAPI staining was used as controls for cell density. Staining of FN matrix in 1143mel and F27mel cells with all four Abs showed these similar patterns: intensive staining of FN in 1143mel cells, but little or weak staining in F27mel cells. 1143 and F27 stand for 1143mel and F27mel, respectively.