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. 2002 Jun 3;195(11):1407–1418. doi: 10.1084/jem.20011063

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Copyright © 2002, The Rockefeller University Press

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Figure 6. — The tolerant cells in TCRxgag are a stable population and not deleted in thymectomized animals. 5-wk-old TCR-TG and TCRxgag mice were anaesthetized and their thymi removed. After 5 wk, splenocytes from control and thymectomized mice were (A) stained with CD8; Vα3 and Vβ12, and after gating on CD8⁺ cells, T cell receptor expression was analyzed. For analysis of lysis by thymectomized mice (B), 1.5 × 10⁷ TCR-TG and TCRxgag splenocytes from normal control (N) or adult-thymectomized (ATx) mice were stimulated with 5 × 10⁶ irradiated FBL cells in the presence of 5 U/ml IL-2. After 3 d, the cells were CD4 depleted and tested in a standard ⁵¹[Cr]-release assay against E10 or E10 pulsed with FMuLVgag. E:T ratio was 50:1.