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. 2002 Jun 17;195(12):1533–1539. doi: 10.1084/jem.20020067

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Copyright © 2002, The Rockefeller University Press

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Figure 5. — Role of IL-7 in supporting survival of CD44^hi CD8⁺ cells in vitro and in vivo in IL-7 TG mice. (A) IL-7 can maintain survival of CD44^hi CD8⁺ cells in vitro. Aliquots of purified IL-7 TG LN T cells were incubated with the indicated cytokines at (20 ng/ml) either alone or in the presence of anti–IL-7Rα mAb (A7R34), anti-CD122 mAb (TM-β1), or both mAbs at 50 μg/ml. The cells were harvested 5 d later and stained for CD4, CD8, and CD44, and the numbers of viable CD44^hi CD8⁺ cells were calculated. (B) Blocking the IL-7R leads to depletion of CD44^hi CD8⁺ cells in IL-7 TG mice. Groups of two to three LMs, IL-7 TG, and IL-15⁻ IL-7 TG mice were injected three times with either 200 μg of anti–IL-7Rα mAb (A7R34) or rat IgG at 2-d intervals. 2 d after the last injection, LN and spleen cells were collected and stained for CD4, CD8, and CD44. The total numbers of CD44^hi CD8⁺ cells recovered from spleens and pooled LN are shown. Another experiment showed similar results.