Table I.
ALCAMhigh Cells Support the Differentiation of Hematopoietic Cells
| Percentage of positive cells
|
|||
|---|---|---|---|
| ALCAMlow/− | ALCAMhigh | ALCAMhigh/osteogenic | |
| CD4 | 1.2 ± 0.5 | 1.0 ± 0.1 | 7.7 ± 1.8 |
| CD8 | 0.3 ± 0.2 | 3.8 ± 0.5 | 6.0 ± 1.2 |
| B220 | 4.9 ± 1.6 | 11.8 ± 2.0 | 1.3 ± 0.3 |
| TER-119 | 2.8 ± 1.0 | 6.1 ± 5.2 | 2.5 ± 0.5 |
| Mac-1 | 76.3 ± 7.7 | 72.3 ± 4.2 | 93.3 ± 2.4 |
| Gr-1 | 83.9 ± 12.1 | 62.3 ± 5.8 | 69.0 ± 1.1 |
BM-derived Lin−c-Kit+Sca-1+ HSCs (500 cells per well) were cocultured with ALCAMlow/−, ALCAMhigh cells, or osteogenic differentiation induced ALCAMhigh cells (ALCAMhigh/osteogenic) in the presence of SCF, IL-6, IL-7, and Epo. After 8 d of cultivation, the expression of lineage markers was analyzed by FACS®. The data shown represents the mean ±SD.