Table I.
Expression and Modulation of Cytokine Receptor Chains in CD4+ Naive and Memory T Cell Subsets
| (A) Cytokine receptor surface expression (MFI)a | |||
|---|---|---|---|
| T naive | TCM | TEM | |
| IL-6R | 14 | 15 | 3 |
| IL-10R | 4 (5)b | 7 (13)b | 7 (13)b |
| (B) αc chain surface expression (fold-induction)c | |||
| TNF-α | 2.5 | 2 | 1.5 |
| IL-6 | 2 | 1.5 | 1 |
| IL-10 | 2 | 2 | 3.5 |
| IL-12 | 1.5 | 1.5 | 2.5 |
| TNF-α + IL-6 + IL-10 | 4 | 3 | 4.5 |
a
Unstimulated T cell subsets were stained with antibodies to IL-6R or IL-10R. Mean fluorescence index (MFI) after subtraction of the background is indicated.
b
Values in parentheses indicate IL-10R expression after stimulation with IL-7 and IL-15 for 16 h.
c
T cell subsets were cultured as in Fig. 4 and analyzed after 16 h for surface expression of ac chain. The fold-increase in MFI after subtraction of the background is shown.