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. 2003 Oct;2(5):1076–1090. doi: 10.1128/EC.2.5.1076-1090.2003

FIG. 2.

FIG. 2.

Alternative versions of chromosomes 2, 3, and 1. (A) Southern blot of a pulsed-field electrophoresis gel of Asp718-digested total DNA, hybridized successively with probe 6R to reveal alternative versions of chromosomes 2 and 3 and with probe 5L to reveal alternative versions of chromosomes 2 and 1. (B) Maps of main alternative versions of macronuclear chromosomes 2, 3, and 1 (mac 2, mac 3, and mac 1, respectively) between Asp718 sites A5 and A6. Almost all of the alternative rearrangements occur between BamHI site B1 and SacI site S. The lengths of the B1-S fragments of the different chromosome 2 versions and of the telomeric fragments of the different versions of chromosomes 3 and 1 are represented by broken lines. Gray boxes show the positions of alternative telomere addition regions. Probes 5L and 6R are shown as black boxes.