Figure 1.

Analysis of Vα14 NKT cells in EAE-susceptible mice. (A) Numbers of CD1d/α-GalCer tetramer-positive cells in EAE-susceptible mice. Liver mononuclear cells from the indicated mice were stained with APC-labeled CD1d/α-GalCer tetramers and FITC-labeled anti-TCR-β antibodies and analyzed by flow cytometry. Numbers indicate the percentage of TCR-β⁺ tetramer⁺ cells. Representative data are shown from three separate experiments. (B) In vitro responses of spleen cells from EAE-susceptible mice to α-GalCer. Splenocytes (2 × 10⁵) from the indicated mice were cultured with 50 ng/ml of α-GalCer. 3 d later proliferation was measured by [³H]thymidine incorporation and cytokine production in the culture supernatant was measured by ELISA. Data represent the mean ± SE of three mice per group. Representative data are shown from three separate experiments.