Nested PCR with the nuclei of the flagellates. Lane 1, primer set I and five nuclei of K. bonita; lane 2, primer set II and five nuclei of K. bonita; lane 3, primer set I and five nuclei of D. nana; lane 4, primer set II and five nuclei of D. nana; lane 5, primer set I and five nuclei of D. operculata; lane 6, primer set II and five nuclei of D. operculata.