Alignment of H. jecorina xylitol dehydrogenase with other xylitol and d-sorbitol dehydrogenases. Dehydogenases were as follows: NcXdh1, N. crassa Xdh1 (NCU00891.1); HjXdh1, H. jecorina Xdh1; CaXdh1, Candida sp. strain HA167 Xdh1 (AAC24597.1); ScSor1, S. cerevisiae Sor1 (NP_012693.1); ScXdh1, S. cerevisiae Xhd1 (NP_013171.1); PsXdh1, P. stipitis Xdh1 (P22144); and SpSdh1, S. pombe Sdh1 (P36624). Diamonds indicate the first zinc binding site (C50, H75, and E161), and asterisks indicate the second zinc binding site (C105, C108, C111, and C119). The dashes over the sequence indicate the zinc-containing alcohol dehydrogenase signature, and the plus signs over the sequence indicate an NADbinding site. Residues in white on a black background are conserved in at least 90% of the proteins; residues in white on a grey background are conserved in 40%.