TABLE 1.

Substrate specificity of recombinant Xdh1 of H. jecorina^a

Substrate	Cosubstrate (mM)	Mean ± SDa
		Km (mM)	Vmax (U/mg of protein)
Xylitol	NAD (0.3)	25 ± 7	0.06 ± 0.02
d-Sorbitol	NAD (0.3)	24 ± 7	0.04 ± 0.01
Galactitol	NAD (0.3)	ND	<0.002 ± 0.001
l-Arabinitol	NAD (0.3)	ND	<0.002 ± 0.001
d-Xylulose	NADH (0.15)	4.5 ± 0.8	0.10 ± 0.03
d-Fructose	NADH (0.15)	400 ± 120	0.15 ± 0.04
d-Galactose	NADH (0.15)	ND	<0.005 ± 0.002
NAD+	Xylitol (50)	0.025 ± 0.010	0.06 ± 0.002
NADH+	d-Xylulose (5)	0.35 ± 0.014	0.10 ± 0.03
NADP+	Xylitol (50)	ND	<0.005 ± 0.001

^aND, not determined.