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. 2002 Feb 4;195(3):359–365. doi: 10.1084/jem.20011576

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Copyright © 2002, The Rockefeller University Press

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Figure 4. — Western blot analysis showing the stability of expression of PspA. Culture supernatants (A) and whole cell sonicates (B) obtained from the last positive nasal or throat culture from each of the colonized subjects (FS#) were compared with the inoculation strain (lane I) by immunoblotting with post-inoculation serum from subject FS31. The arrow indicates the position of the PspA_31–189. The isolate recovered from one of the subjects (FS162) at day 3 postinoculation was found to be a type 6A strain expressing a larger full-length PspA. This subject was then considered in the uncolonized group. Size markers are in kilodaltons.