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. 2002 Feb 4;195(3):327–333. doi: 10.1084/jem.20010938

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Copyright © 2002, The Rockefeller University Press

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Figure 3. — IL-2–activated NK cells induce DC maturation. (A and B) DC were cultured for 18 h in medium only or in the presence of LPS, IL-2–activated [NK(IL-2)] or untreated [NK(medium)] NK cells. (A) Cells were washed and DC expression of CD86, CD83, CD80, HLA-DR, and CCR7 was analyzed by immunofluorescence. (B) DC, depleted of NK cells and irradiated, were cultured for 5 d with allogeneic naive CD4⁺ PBL, and ³H-TdR uptake was determined. (C) CD86 expression was determined on DC after an 18-h culture in the absence of any stimulus, or with PBL, or NK-depleted PBL (PBL–NK), either IL-2–activated or untreated.