Figure 1.

FHA inhibits DC IL-12 and CCL3 production by inducing IL-10. (A) Bone marrow–derived DCs were stimulated with FHA alone or with FHA after 2 h with LPS (1 μg/ml) and IFN-γ (20 ng/ml). After 24 h of culture, supernatants were removed and IL-12p70 and IL-10 were determined by specific immunoassays. (B) DCs were preincubated with FHA (5 μg/ml) with or without anti–IL-10, followed 2 h later with LPS (1 μg/ml) and IFN-γ (20 ng/ml). After 24 h of culture, supernatants were removed and IL-12p70, IL-12p40, CCL3, and IL-10 determined by specific immunoassays. Results are representative of six experiments.