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. 2002 Jan 21;195(2):221–231. doi: 10.1084/jem.20011288

Figure 4.

Figure 4.

Tr1 clones inhibit cytokine production by Th1 but not Th2 clones. (A) PRN-specific Tr1 clone TEK.1 (105/ml) and influenza virus HA-specific Th1 clone HDS2.6 (105/ml) were cultured alone or together with PRN (5 μg/ml) and/or influenza virus (10 HAU/ml) and splenic APCs (2 × 106/ml). Supernatants were removed after 2–3 d and concentrations of IL-4, IL-5, IL-10, and IFN-γ determined by immunoassays. In the cocultures, Tr1 and Th1 cells were cultured in the same well, HDS2.6 plus TEK.1, or separated by a semi-permeable membrane, where supernatants were sampled from the Th1 side, HDS2.6 (TEK.1) or the Tr1 side, TEK.1 (HDS2.6). *P < 0.05, **P < 0.01, ***P < 0.001 HDS2.6 alone versus cocultured with TEK.1. (B) PRN-specific Tr1 clone TEK.1 was cultured with antigen alone or with PT-specific Th2 clone PT1.1 or PRN-specificTh2 clone PRN2 and cytokine production assessed by immunoassay. Results are representative of two experiments.