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. 2002 Jan 21;195(2):221–231. doi: 10.1084/jem.20011288

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Copyright © 2002, The Rockefeller University Press

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Figure 8. — FHA stimulates DCs to direct naive T cells to a Tr1 subtype. OVA-specific CD4⁺ T cell lines were generated in vitro by stimulating purified naive T cells from DO11.10 TCR Tg mice with DCs preincubated for 24 h with OVA peptide (0, 0.5, 5, 50 μg/ml) and FHA (5 μg/ml), CpG-ODN (1 μg/ml), FHA, and anti–IL-10 (10 μg/ml) or medium only. After two rounds of antigen stimulation supernatants were removed and concentrations of cytokines determined by immunoassay. Results are representative of two experiments.